![]() ![]() Intensive use of site-specific fungicides leads to the development of fungicide resistance in A. Several fungicides are used to control the disease, to increase productivity, and to improve the storage life of harvested crops. alternata causes black pit on tubers resulting in post-harvest losses of 10% 4. The disease has the potential to reduce the yield up to 30% and up to 70–80% losses are also reported when the disease is left uncontrolled 2, 3. The symptoms include small circular brown lesions which appear on the abaxial sides of leaves. Brown spot, commonly known as “the other early blight”, is an important disease of potato 1. Among these phytopathogenic fungi is Alternaria alternata causing brown spot disease in potato crops. Plant pathogenic fungi destroy a third of all food crops annually, causing economic loss and impacting global poverty. Taken together, this droplet-based microfluidic platform provides a wide range of potential applications for the analysis of fungicide resistance development as well as combinatorial screening of other antimicrobial agents and even antagonistic fungi. The percentage inhibitions assessed by the droplet-based platform were equivalent with those obtained by the other two methods, and the Pearson correlation analysis showed high correlation between the three assays. The efficacy results of the droplet-based antifungal analysis were compared and validated with the results obtained from conventional protocols. ![]() The viability was demonstrated over a period of 24 h and the antifungal screening was achieved using Kunshi/Tezuma as antifungal agent. A droplet-based viability assay was developed, allowing the germination and hyphal growth of single A. In this study, we present a droplet-based microfluidic platform that enables antifungal analysis of single spores of filamentous fungus Alternaria alternata. ![]() The conventional antifungal screening techniques, such as water agar and 96-well plates, are based on laborious protocols and bulk analysis, restricting the analysis at the single spore level and are time consuming. Fungicides are extensively used in agriculture to control fungal pathogens which are responsible for significant economic impact on plant yield and quality. ![]()
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